Abstract
We report the draft genome sequence of Cupriavidus sp. strain SK-3, which can use 4-chlorobiphenyl and 4-clorobenzoic acid as the sole carbon source for growth. The draft genome sequence allowed the study of the polychlorinated biphenyl degradation mechanism and the recharacterization of the strain SK-3 as a Cupriavidus species.
Highlights
Polychlorinated biphenyls (PCB) are pollutants that are difficult to remove from contaminated sites due to their low capacity for degradation and their bioaccumulation in the environment [1, 2]
Based on the Rapid Annotation using Subsystem Technology (RAST) annotation, the SK-3 genome showed an enrichment of genes related to PCB degradation, such as those for biphenyl-degrading enzymes and dioxygenases [12]
A previous study classified SK-3 as a Burkholderia sp. based on biochemical results [1], our analysis based on the 16S rRNA gene phylogeny strongly showed that SK-3 belongs to the Cupriavidus genus, as SK-3 is tightly clustered with Cupriavidus bacteria and clearly separated from Ralstonia and Burkholderia bacteria on the phylogenetic tree
Summary
Polychlorinated biphenyls (PCB) are pollutants that are difficult to remove from contaminated sites due to their low capacity for degradation and their bioaccumulation in the environment [1, 2]. Strain SK-3, originally isolated from PCB-contaminated tertiary lagoon sludge, grows on both 4-chlorobiphenyl (4-CB) and 4-clorobenzoic acid (4-CBA) as the sole carbon source [1, 2]. The total number of reads was 10,024,280, with a total length of 511,238,280 bp (about 53-fold coverage of the estimated genome size).
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