Abstract
A draft genome sequence of “Cohnella kolymensis” strain B-2846 was derived using IonTorrent sequencing technology. The size of the assembly and G+C content were in agreement with those of other species of this genus. Characterization of the genome of a novel species of Cohnella will assist in bacterial systematics.
Highlights
Strain 20.16 (VKM B-2846) was isolated from the permafrost using aseptic sampling technique in the borehole drilled in the Duvaniy Yar area, Siberia
According to the results of phylogenetic analysis based on 16S rRNA gene sequences, strain B-2846 is most closely related to Cohnella spp., with Cohnella collisoli showing the highest level of similarity (96.91%)
Based on the threshold of 98.65% in recognizing novel species [2], strain B-2846 is regarded as a novel species of Cohnella with the proposed name “Cohnella kolymensis.”
Summary
Strain 20.16 (VKM B-2846) was isolated from the permafrost using aseptic sampling technique in the borehole drilled in the Duvaniy Yar area, Siberia. According to the results of phylogenetic analysis based on 16S rRNA gene sequences, strain B-2846 is most closely related to Cohnella spp., with Cohnella collisoli showing the highest level of similarity (96.91%). The sequencing reads were generated by IonTorrent PGM using a 314v2 chip and a 400-bp sequencing kit. Assemblies of reads generated by SPADES plugin ver.
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