Abstract
We announce the draft genome sequence of Calothrix strain 336/3, an N2-fixing heterocystous filamentous cyanobacterium isolated from a natural habitat. Calothrix 336/3 produces higher levels of hydrogen than Nostoc punctiforme PCC 73102 and Anabaena strain PCC 7120 and, therefore, is of interest for potential technological applications.
Highlights
To diversify a potential to exploit naturally occurring photosynthetic organisms for sustainable biofuel production, we investigated strains from the University of Helsinki Culture Collection (UHCC) for their capability to produce H2 [1]
DNA preparations were obtained as described by Neilan et al [4] and sent for commercial sequencing at the Beijing Genome Institute (BGI), where two Calothrix 336/3 DNA libraries containing short ~500-bp inserts and mate-pair ~2,000-bp inserts were constructed
The taxonomic analysis with MEGAN v. 5.5.4 [6] revealed that the first group of 27 scaffolds contained proteins that were found in other cyanobacterial species, while the second group belonged to an unknown Bacteroides-like genus, which resulted, most probably, from strain contamination
Summary
To diversify a potential to exploit naturally occurring photosynthetic organisms for sustainable biofuel production, we investigated strains from the University of Helsinki Culture Collection (UHCC) for their capability to produce H2 [1]. After optimization of the production system and exclusion of strains that had a toxic nature or have been too light sensitive, two strains, Calothrix 336/3 and Calothrix XPORK 5E, have been found to be the best H2-producing cyanobacteria among investigated species [1, 2]. Calothrix 336/3 is an N2-fixing heterocystous filamentous cyanobacterium isolated from the Enäjärvi lake, Laukilanlahti, Finland [3].
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