Abstract
Despite its global abundance, Bacillus pumilus is poorly studied. The Fairview strain was obtained from a methanogenic anaerobic coal digester. The draft genome sequence was 3.8 Mbp long and contained 3,890 protein-coding genes. Like the SAFR-032 strain, it includes B. pumilus-specific proteins that likely confer enhanced resistance to environmental stresses.
Highlights
Despite its global abundance, Bacillus pumilus is poorly studied
Bacillus pumilus Fairview was isolated from a 45°C methanogenic anaerobic digester sample obtained from a coal seam gas formation water sample growing with a highly volatile bituminous Bowen Basin coal as the feedstock
The formation water was sourced from the Fairview coal seam gas field near Injune, Queensland, Australia (S25°51; E148°34) from a borehole intersecting a coal seam at approximately 600 m subsurface
Summary
Bacillus pumilus is poorly studied. The Fairview strain was obtained from a methanogenic anaerobic coal digester. Bacillus pumilus Fairview was isolated from a 45°C methanogenic anaerobic digester sample obtained from a coal seam gas formation water sample growing with a highly volatile bituminous Bowen Basin coal as the feedstock. The formation water was sourced from the Fairview coal seam gas field near Injune, Queensland, Australia (S25°51; E148°34) from a borehole intersecting a coal seam at approximately 600 m subsurface. These efforts included dilution on a range of media, including peptone, yeast-extract, and glucose (PYG) (all 1 g liter–1) agar [1], followed by incubation to isolate facultative aerobes.
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