Abstract
ABSTRACTBacillus aryabhattai PHB10 is a poly(3-hydroxybutyrate) (PHB)-accumulating bacterium isolated from domestic sewerage. Here, we report the 4.19-Mb draft genome sequence, with 4,050 protein-coding genes and a G+C content of 37.5%. This sequence will be helpful in the study of the high-level PHB accumulation mechanism of the strain.
Highlights
Bacillus aryabhattai PHB10 is a poly(3-hydroxybutyrate) (PHB)-accumulating bacterium isolated from domestic sewerage
Genomic DNA was isolated from strain PHB10 using a Wizard genomic DNA purification kit (Promega, WI) according to the manufacturer’s instructions
The polyhydroxyalkanoic acid biosynthesis gene cluster was identified with the genes phaP, phaQ, phaR, phaB, and phaC [poly(R)-hydroxyalkanoic acid synthase subunit C]
Summary
Bacillus aryabhattai PHB10 is a poly(3-hydroxybutyrate) (PHB)-accumulating bacterium isolated from domestic sewerage. Genomic DNA was isolated from strain PHB10 using a Wizard genomic DNA purification kit (Promega, WI) according to the manufacturer’s instructions. A barcoded genomic library was generated using an Ion PGM library preparation kit and loaded into a 318 v2 chip (Life Technologies, Inc., CA).
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