Abstract
Bacillus amyloliquefaciens strain RHNK22 isolated from groundnut rhizosphere showed direct and indirect plant growth-promoting traits along with biosurfactant activity and reduction in surface tension of water. Biosurfactants were identified as lipopeptides (surfactin, iturin, and fengycin) by molecular and biochemical analysis in our studies.
Highlights
Bacillus amyloliquefaciens strain RHNK22 was isolated from groundnut by the enrichment culture method as described by Dubey and Juwarkar (1)
B. amyloliquefaciens sp. plantarum CAUB946 is the most predominant strain among B. amyloliquefaciens species in the BLAST result which is used for single nucleotide polymorphism (SNP) analysis
SNP discovery was carried out using the draft genome identified through BLAST
Summary
Bacillus amyloliquefaciens strain RHNK22 was isolated from groundnut by the enrichment culture method as described by Dubey and Juwarkar (1). B. amyloliquefaciens RHNK22 has been used to produce industrially important biosurfactants with surface tension reduction and antifungal activity (2), plant growthpromoting traits, and antioxidant and probiotic properties (unpublished data) and was subjected to genomic sequencing. The shotgun sequencing of Bacillus amyloliquefaciens strain RHNK22 genome was performed using a paired-end (PE) 2 ϫ 150 bp library on the Illumina platform (NextSeq 500). Coding DNA sequence (CDS) prediction was performed using Prodigal v2.60.
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