Abstract

Here we report the draft genome sequence of an Escherichia coli O157:H43 strain, designated T22, with an atypical virulence gene profile and isolated from healthy cattle. T22 produces cytolethal distending toxin V (CDT-V) and belongs to phylogenetic group B1 and sequence type 155 (ST155).

Highlights

  • We report the draft genome sequence of an Escherichia coli O157:H43 strain, designated T22, with an atypical virulence gene profile and isolated from healthy cattle

  • We identified atypical E. coli O157:H43 strains from healthy cattle that harbor neither stx nor eae virulence genes but that produce cytolethal distending toxin V (CDT-V), carry the genes encoding long polar fimbriae, and belong to phylogenetic group B1 [7, 8]

  • The chromosome and the plasmid DNA was sequenced and assembled using the combination of three next-generation sequencing (NGS) approaches

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Summary

Introduction

We report the draft genome sequence of an Escherichia coli O157:H43 strain, designated T22, with an atypical virulence gene profile and isolated from healthy cattle. Enterohemorrhagic Escherichia coli (EHEC) strain O157:H7 has become a significant food-borne pathogen since its discovery [1]. Strains of the serotype O157:NM (nonmotile) caused severe outbreaks in Europe [2]. Typical EHEC O157 strains produce Shiga toxin (Stx) and carry the locus of enterocyte effacement (LEE) pathogenicity island encoding the adhesin intimin [3].

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