Abstract
We report the 3.7-Mb draft genome of Acinetobacter oleivorans strain PF1, a hydrocarbonoclastic Gram-negative bacterium in the class Gammaproteobacteria, isolated from poplar trees growing on a diesel-contaminated plume at the Ford Motor Company site in Genk, Belgium. Strain PF1 is a potent plant-growth promoter, useful for diesel fuel phytoremediation applications.
Highlights
Acinetobacter sp. strains are known to utilize diesel fuel and other recalcitrant organics as carbon and energy sources (1)
Acinetobacter oleivorans strain PF1, isolated from poplar trees growing on a diesel-contaminated plume, was shown, using GCMS, to degrade 41% of 1.0·g·literϪ1 diesel fuel over 10 days
Genomic DNA of PF1 was extracted with a Qiagen blood and tissue kit (Qiagen NV, Hilden, Germany) prior to enzymatic digestion and ligation of sequencing adaptors using an Ion Xpress Plus fragment library kit (Life Technologies Inc., Burlington, ON, Canada)
Summary
Acinetobacter sp. strains are known to utilize diesel fuel and other recalcitrant organics as carbon and energy sources (1). Acinetobacter oleivorans strain PF1, isolated from poplar trees growing on a diesel-contaminated plume, was shown, using GCMS, to degrade 41% of 1.0·g·literϪ1 (wt/vol) diesel fuel over 10 days. Partial 16S rRNA gene sequence data and phenotypic profiling indicated that PF1’s closest relative is Acinetobacter sp.
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