Abstract
The 3.94-Mb draft genome of Acinetobacter calcoaceticus GK1, a hydrocarbonoclastic plant growth-promoting Gram-negative rhizospheric bacterium, is presented here. Isolated at the Ford Motor Company site in Genk, Belgium, from poplar trees planted on a diesel-contaminated plume, GK1 is useful for enhancing hydrocarbon phytoremediation.
Highlights
The presence of Acinetobacter sp. strains in environments contaminated with pollutants such as diesel fuel, crude oil, phenol, and other recalcitrant organics has been well documented [1,2,3,4,5]
Acinetobacter calcoaceticus GK1 was isolated from the rhizosphere of poplar trees in a diesel-contaminated environment
Genomic DNA of GK1 was extracted with a Qiagen blood and tissue kit (Qiagen NV, Hilden, Germany) and an IonTorrent PGM was used to generate a whole-genome shotgun sequence using the methods described in reference 6
Summary
The presence of Acinetobacter sp. strains in environments contaminated with pollutants such as diesel fuel, crude oil, phenol, and other recalcitrant organics has been well documented [1,2,3,4,5]. Strains in environments contaminated with pollutants such as diesel fuel, crude oil, phenol, and other recalcitrant organics has been well documented [1,2,3,4,5]. Acinetobacter calcoaceticus GK1 was isolated from the rhizosphere of poplar trees in a diesel-contaminated environment. Phenotypic profiling and partial 16S rRNA gene sequence data showed that GK1’s closest relative is Acinetobacter calcoaceticus PHEA2
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