Abstract
Here we report the 3.7-Mb draft genome sequence of Acetobacter tropicalis NBRC16470T, which can produce optically pure d-glyceric acid (d-GA; 99% enantiomeric excess) from raw glycerol feedstock derived from biodiesel fuel production processes.
Highlights
We report the 3.7-Mb draft genome sequence of Acetobacter tropicalis NBRC16470T, which can produce optically pure D-glyceric acid (D-GA; 99% enantiomeric excess) from raw glycerol feedstock derived from biodiesel fuel production processes
We previously reported a biotechnological method for producing optically pure D-glyceric acid (D-GA) with a 99% enantiomeric excess from glycerol using Acetobacter tropicalis NBRC16470T
Genomic sequence assembly using the SOAPdenovo2 assembler [8] generated 453 scaffolds composed of 654 contigs and a 3.7-Mb draft genome sequence at 151-fold coverage from the paired-end library
Summary
We report the 3.7-Mb draft genome sequence of Acetobacter tropicalis NBRC16470T, which can produce optically pure D-glyceric acid (D-GA; 99% enantiomeric excess) from raw glycerol feedstock derived from biodiesel fuel production processes. Acetic acid bacteria (Acetobacteriaceae) are obligate aerobes that show efficient oxidation of a wide range of substrates, such as alcohols, sugars, sugar acids, and sugar alcohols. These bacteria have numerous membrane-bound dehydrogenases and oxidoreductases to accomplish such oxidative reactions; energy-consuming transport of substrates into the cell and products out of the cell is not required [1]. Have been used for industrial vinegar production due to their strong ethanol-oxidizing ability, and their complete or draft genome sequences were reported previously (e.g., 2, 3).
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