Draft Genome Sequence of a Community-Acquired Methicillin-Resistant Staphylococcus aureus USA300 Isolate from a River Sample.

Sarah Lepuschitz,Werner Ruppitsch,Franz Allerberger,Robert Mach,Burkhard Springer

doi:10.1128/genomea.01166-17

Abstract

ABSTRACTThe increasing emergence of multiresistant bacteria in health care settings in the community and in the environment represents a major health threat worldwide. Here, we report the draft genome sequence of a community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) USA300 isolate (W1) from a small river in southern Austria.

Highlights

The increasing emergence of multiresistant bacteria in health care settings in the community and in the environment represents a major health threat worldwide
Whole-genome sequence analysis of this water isolate, W1, identified the main characteristics of community-acquired methicillin-resistant Staphylococcus aureus (MRSA) (CA-MRSA) USA300: sequence type 8 (ST8), spa type t008, staphylococcal cassette chromosome mec element type IV (SCCmec IV), Panton-Valentine leukocidin (PVL), and the arginine catabolic mobile element (ACME) cluster. This is the first report and draft genome sequence of a MRSA USA300 isolate derived from a water sample
For the isolation of S. aureus from water samples, 100-ml aliquots were filtrated, and filters were incubated in thioglycolate at 37°C overnight

Summary

Introduction

The increasing emergence of multiresistant bacteria in health care settings in the community and in the environment represents a major health threat worldwide. In 2016, the Austrian Agency for Health and Food Safety started a pilot project to investigate antimicrobial resistance (AMR) in surface water. Among several water samples from diverse Austrian rivers, one sample from a small river in the province Carinthia contained a MRSA isolate. Whole-genome sequence analysis of this water isolate, W1, identified the main characteristics of community-acquired MRSA (CA-MRSA) USA300: sequence type 8 (ST8), spa type t008, staphylococcal cassette chromosome mec element type IV (SCCmec IV), Panton-Valentine leukocidin (PVL), and the arginine catabolic mobile element (ACME) cluster.

Results

Conclusion