Abstract
We announce here the genome sequencing of Pseudomonas sp. strain 11/12A from Lake Washington sediment. From the genome content, a versatile lifestyle is predicted but not one of bona fide methylotrophy. With the availability of its genomic sequence, Pseudomonas sp. 11/12A presents a prospective model for studying microbial communities in lake sediments.
Highlights
When natural microbial communities from Lake Washington are incubated under the atmosphere of methane, simple and semistable communities are formed consisting of bona fide methanotroph species and of nonmethanotrophic satellite species
Pseudomonas sp. strain 11/12A was isolated from such an enrichment culture that was incubated at 10°C in a minimal salts medium, with multiple transfers and dilutions, for approximately 18 months (1), by plating onto nutrient broth (NB) agar medium (Difco)
The draft genome of Pseudomonas sp. 11/12A was generated at the Department of Energy (DOE) Joint Genome Institute (JGI), Walnut Creek, CA, USA, using the Pacific Biosciences (PacBio) sequencing technology (2)
Summary
When natural microbial communities from Lake Washington are incubated under the atmosphere of methane, simple and semistable communities are formed consisting of bona fide methanotroph species and of nonmethanotrophic satellite species. Some of the types found to persist in such methane-fed microcosms are the Pseudomonas species (1). Strain 11/12A was isolated from such an enrichment culture that was incubated at 10°C in a minimal salts medium, with multiple transfers and dilutions, for approximately 18 months (1), by plating onto nutrient broth (NB) agar medium (Difco).
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