Abstract
The fractionation of rat-liver RNA was studied by direct ultraviolet densitometry of dry agar electrophoregrams. Highly reproducible electrophoretic patterns of phenol extracted RNA and residual RNA were obtained. Phenol-extracted RNA was resolved into 3 main and 4–6 minor fractions. The main fractions accounted in most cases for 90 % of the absorbancy area and corresponded to the two components of ribosomal RNA and soluble RNA. Residual RNA extracted with phenol at 65° in the presence of sodium dodecylsulphate was resolved into 11–13 fractions, 3 of them being components of ribosomal RNA and low-molecular-weight RNA. A slow-moving region was also present in which the two slowest components are extracted only in the presence of sodium dodecylsulphate.
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