Abstract
RNA isolated from the 600 x g supernatant of rat liver homogenate by means of a cold phenol-SDS procedure was fractionated by a preparative agar gel electrophoretic technique. The base composition of the two ribosomal RNA components and of s-RNA was determined from the UV-absorbancy data, as well as from the radioactivity of the four 2′(3′)-mononucleotides after different labeling times with 32p. The two ribosomal RNA components had statistically significant differences in their base composition, the lighter component showing a lower G and C content. The base composition position of the newly synthesized RNA differed from that of the total RNA in both ribosomal RNA components, having a lower G+ C a+ U ratio. This ratio approached that of DNA at 1 hr, and was close to that of ribosomal RNA at 4.5 hr labelling time. The conclusion is drawn that in rat liver cytoplasm a particular RNA fraction is present, which shows: a/ higher rate of turn-over; b/ electrophoretic mobility in agar gel exactly coinciding with that of the corresponding peaks of the ribosomal RNA, and c/ base composition significantly different from that of the ribosomal RNA.
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