Die Polymerisation von 4-Thiouridin-5′-diphosphat und 4-thiothymidin-5′-diphosphat durch polynucleotidphosphorylase aus Micrococcus lysodeikticus

Abstract

Listen

Translate article

Abstract Polymerization of 4-thiouridine 5′-diphosphate and 4-thiothymidine 5′-diphosphate by polynucleotide phosphorylase from Micrococcus lysodeikticus 4-Thiouridine 5′-diphosphate (s 4 UDP) and 4-thiothymidine 5′-diphosphate were polymerized by polynucleotide phosphorylase from Micrococcus lysodeikticus only in the presence of UDP, ADP, IDP, or CDP. The copolymerization of s 4 UDP and UDP was studied in detail. It was found, that the polymerization was inhibited by increasing amounts of s 4 UDP. Since UDP polymerization could be inhibited by addition of poly (U 1.25 , s 4 U), it is supposed that the copolymerization of s 4 UDP and UDP is inhibited by polynucleotides poly (U, s 4 U). 4-Thiouridylyl-(3′-5′)-4-thiouridine and uridylyl-(3′-5′)-4-thiouridine have been found to stimulate the poly U synthesis depending on the primer. Polynucleotides of the general formula poly (U, s 4 U) were isolated on a preparative scale. Their compositions could be calculated from ultraviolet absorption spectra.