Development of Silver Nanoparticles-Loaded Chitosan/PVA Gel

Abstract

Polymer-based hydrogels are suitable for development as wound dressings because the hydrogel can regulate the amount of fluid and moisture in the wound area, exhibit a network structure, and be compatible with the human body. Therefore, this work focused on the preparation of 2% w/v (Chitosan:CS)/2%,4%,6% w/v (Poly(vinyl alcohol):PVA) gel containing silver nanoparticles for antibacterial use, synthesized by the chemical crosslinking method with glutaraldehyde. Silver nanoparticles were prepared at a concentration of 1 mmol with black lemon extract as a reducing agent and a stabilizer packaged in the gel. From the study of absorbance, particle size, and morphology, it was found that silver nanoparticles were 10.60± 2.20 nm, with good dispersion and spherical shape. The swelling behavior of 2% w/v CS/2% w/v PVA gel showed the highest swelling at 95.74±21.29%. In addition, 2% w/v CS/2% w/v PVA gel containing silver nanoparticles could inhibit the growth of gram-positive and gram-negative bacterial microorganisms. Thus 2% w/v CS/2% w/v PVA gel containing silver nanoparticles is suitable for medical applications.