Abstract
The present study explores the in situ fabrication of chitosan-poly(vinyl alcohol)-silver nanocomposite films in view of their increasing applications as antimicrobial packaging, wound dressing and antibacterial materials. The reduction of silver ions into silver nanoparticles (AgNPs) is achieved in acidic solution of chitosan (C) and poly (vinyl alcohol) (PVA) using their functional groups (-OH, -COOH, -NH2 groups). The presence of silver nanoparticles in the chito-san-PVA film is confirmed by UV-Vis spectroscopy, Fourier Transform Infrared (FTIR) spectroscopy and X-ray Dif-fraction (XRD) analysis. The Scanning Electron Microscopic (SEM) images illustrate the presence of embedded silver nanoparticles throughout the films. In addition, the formed silver nanoparticles have an average particle size of ~ 16.5 nm as observed by Transmission Electron Microscopy (TEM). The anti-microbial and anti-fungal activity of the chitosan-PVA silver nanoparticle films have demonstrated significant effects against Escherichia coli (E. coli), Pseudomonas, Staphylococcus, Micrococcus, Candida albicans, and Pseudomonas aeruginosa (P. aeruginosa). To improve further their therapeutic efficacy as anti-microbial agents, curcumin encapsulated chitosan-PVA silver nanocomposite films are developed which showed enormous growth inhibition of E. coli compared to curcumin and chitosan-PVA silver nanoparticles film alone. Therefore, the present study clearly provides novel antimicrobial films which are potentially useful in preventing/treating infections.
Highlights
Human beings are often infected by micro-organisms such as bacteria, yeast, mold, virus, etc [1]
In order to study the release of curcumin from loaded films, known weights were placed in a measured volume (50ml) of 7.4 pH phosphate buffer at room temperatures and the released amount of curcumin was determined at different time intervals by recording the absorbance of release medium by UV-Vis spectrophotometer
Due to greater importance of chitosan and Polyvinyl alcohol (PVA) in biomedical applications, chitosan-PVA silver nanoparticle films are developed via green process
Summary
Human beings are often infected by micro-organisms such as bacteria, yeast, mold, virus, etc [1]. Tripathi et al [23] prepared chitosan-PVA blend films for food packaging applications and they have shown to possess antimicrobial activity against food pathogenic bacteria. Curcumin (CUR), a hydrophobic polyphenolic compound derived from the rhizome of the herb curcuma longa, possesses a wide range of biological activities including wound healing, anti-bacterial, anti-oxidant, anti-inflammatory and anti-cancer properties. This compound is incorporated into chitosan-PVA nanoparticles film to improve significantly the therapeutic antibacterial efficacy of the film
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
