Development Of Mutant From Lemon Leaves By Using Gamma Radiation And Leaf Cut Method Under Glass House And Field Condition

Abstract

An experiment was conducted at the Horticulture division of Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh during the period from March 2024 to September 2024 to develop mutants from lemon leaves by using gamma radiation and leaf cut method under glasshouse and field conditions to find out the appropriate combination of irradiation dose and suitable environment on success, survivability, and growth of lemon. The experiment was consisted of two factors such as Environmental condition: (i) glass house and field condition and (ii) Nine gamma radiation levels (0 GY, 60 GY,80Gy,100Gy, 120 GY,140 GY,160 GY,180 GY and 200 GY).Mature leaves were collected from BINA Lebu-1 mother plants.Collected leaves were exposed by different doses of gamma radiation separately with 60 GY, 80GY, 100 GY, 120 GY, 140 GY, 160 GY,180 GY, 200 GY, and also a control at BINA from 60Co gamma radiation sources. The experiment was laid out in RCBD with three replications. Total 18 treatment combinations of the environmental condition and radiation levels. Irradiated leaves of lemon were planted in the unit plots on 8 March 2023 in the afternoon with spacing of 15cmx15cm (row to row and leaf to leaf distance). Different environmental condition showed significantly influenced on almost all the mentioned parameters studied concerned with growth and growth contributing characteristics. The highest percentage of success and survivability was found in glass house condition and the lowest % success and %survivability was found in field condition.The glasshouse condition producedhigher number of roots(4.77), longer root length(9.24cm), diameter of shoot( 4.95mm), higher number of leaves per plant(50.66) and field condition produced lower number of roots(3.55), smallest longer root length(7.14cm), lower diameter of shoot(2.26mm), lower number of leaves(43.00) per plant compared to the field condition. The application of 120GY irradiation level produced the highest number of roots (8.50), longest root length (16.1cm),the highest diameter of shoot (5.65mm), andthe highest number of leaves per plant (70.00) as compared to control. The combined effect of environmental condition and gamma radiations was statistically significant on the success, survivability and growth.120GY irradiation level in glasshouse condition produced the maximum roots (7.00), the longest root length (17.5.cm), the largest diameter of shoot (4.80mm), highest canopy volume (0.12m3 ) and the maximum leaves per plant (73.00). Similarly, the lowest values were in respect of almost all the mentioned parameters were found at field condition with control treatment. Therefore, the overall results indicate that glasshouse condition with 120 GY might be an efficient and eligible practice for developing mutant from leaves