Effect Of Different Doses Of IBA On Success, Survivability And Growth Of Lemon Using Leaf Cut Method Under Glass House And Field Condition

Abstract

An experiment was conducted at the Horticulture division of Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh during the period from March, 2024 to June, 2024 to evaluate the quick propagation techniques from lemon leaves by using IBA under glass house and field condition. The experiment was consisted of two factors such as Environmental condition: (i) glass house and field condition and (ii) Different doses of IBA: D0 (Control), D1 (1000ppm IBA for 3mins) D2 (1500ppm IBA for 3mins) and D3 (2000ppm IBA for 3mins). Mature leaves were collected from BINA Lebu-1 mother plants. Collected leaves were exposed by different doses of IBA solution separately and also a control. The experiment was laid out in RCBD with three replications. Total 24 treatment combinations of the environmental condition and IBA levels. IBA exposed leaves of lemon were planted in the unit plots on 8 March, 2024 in the afternoon with spacing of 15cmx15cm (row to row and leaf to leaf distance). Different environmental condition showed significantly influenced on almost all the mentioned parameters studied concerned with growth and growth contributing characteristics. The highest percentage of success (66.34%), survivability (56.86 and %) and canopy volume (0.055m3) were found in glass house condition and the lowest % success (50.35%) % survivability ( 44.26%) and canopy volume (0.028m3) were found in field condition. The glasshouse condition took the minimum time for root and shoot (43.22 and 70.82 days respectfully) initiation. The application of 1500ppm IBA for 3mins took the minimum time for root and shoot (21.83 and 31.00 days respectfully) initiation and the maximum success ( 97.70 %), survivability (92.77%) and canopy volume (0.088m3).The maximum time took for root and shoot (51.08 and 66.08 days respectfully) initiation and the minimum success ( 48.35 %), survivability (45.59%) and canopy volume (0.008m3) were found from control treatment. The combined effect of environmental condition and IBA was statistically significant on the success, survivability and growth of lemon. The treatment in glasshouse condition with 1500ppm IBA solution for 3mins took the minimum time for root and shoot (18.00 and 51.44 days respectfully) initiation and the maximum success (98.70 %), survivability (96.90%) and canopy volume (0.015m3) as compare to control. Therefore, the overall results indicate that glasshouse condition with 1500ppm IBA solution for 3 mins might be an efficient and eligible practice for developing mutant from leaves. Therefore, the overall results indicate that glasshouse condition with 1000ppm IBA solution for 3mins might be an efficient and eligible practice for quick propagation techniques from leaves