Abstract
A rapid, accurate and sensitive high-performance liquid chromatographic assay was developed for the determination of nifedipine in human plasma. A toluene extract of an alkalinized plasma sample was chromatographed on a reversed-phase column with electrochemical detection at +0.95 V. The recovery of nifedipine from plasma was about 100%. The detection limit for nifedipine in plasma was 2 ng/ml using 0.5 ml of sample. The assay gave a linear response over the concentration range 5–400 ng/ml in plasma. The coefficients of variation from 9.6 to 191.0 ng/ml varied between 5.2 to 1.0% and the accuracy did not exceed 3.0%. Photodegradation products and metabolites of nifedipine did not interfere in the analysis. This method allowed the behaviour of nifedipine in humans to be studied.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call