Abstract
The specific binding between Cy5-labeled goat anti-mouse ImmunoglobulinG (IgG) and mouse IgG with a concentration range from 625 to104 µg ml − 1 has been detected successfully by the oblique-incidence reflectivity difference (OI-RD)method in each procedure of microarray fabrication. The experimental data prove that theOI-RD method can be employed not only to distinguish the different concentrations inlabel-free fashion but also to detect the antibody–antigen capture. In addition, thedifferential treatment of the OI-RD signals can decrease the negative influences of glassslide as the microarray upholder. Therefore the OI-RD technique has promisingapplications for the label-free and high-throughput detection of protein microarrays.
Published Version
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