Label-free and real-time detection of specific binding of IgG proteins by oblique-incidence reflectivity difference method

Yue Sun,Liping He,Yueliang Zhou,Kuijuan Jin,Guozhen Yang,Jingyi Wang,Jun Dai,Shufang Wang,Huibin Lü

doi:10.1007/s11434-012-5263-3

Abstract

The entire interaction process between anti-mouse immunoglobulin-G (IgG) and mouse IgG with a concentration range from 1 to 0.25 mg/mL has been label-free detected by the oblique incidence reflectivity difference (OIRD) method. We achieved the two-dimensional scanning images of OIRD Re{Δp-Δs} intensities before and after interactions as well as the curves of reaction dynamic processes. The experimental results suggest that OIRD method not only can label-free distinguish the different concentrations of proteins and detect biological microarrays, but also can real-time monitor the kinetic processes of biomolecular interactions.

Highlights

The entire interaction process between anti-mouse immunoglobulin-G (IgG) and mouse IgG with a concentration range from 1 to 0.25 mg/mL has been label-free detected by the oblique incidence reflectivity difference (OIRD) method
The experimental results suggest that OIRD method can label-free distinguish the different concentrations of proteins and detect biological microarrays, and can real-time monitor the kinetic processes of biomolecular interactions
We have reported the label-free detection of biomolecular microarrays and real-time detection of biomolecular interactions using OIRD method [8,9,10,11,12,13,14]

Summary

Introduction

SPECIAL TOPICS: Label-free and real-time detection of specific binding of IgG proteins by oblique-incidence reflectivity difference method The entire interaction process between anti-mouse immunoglobulin-G (IgG) and mouse IgG with a concentration range from 1 to 0.25 mg/mL has been label-free detected by the oblique incidence reflectivity difference (OIRD) method.

Results

Conclusion