Abstract

Melon (Cucumis melo L.) is one of excellent horticultural commodities in Indonesia. The seed's demand rises in every year due to the increase of people's avocation. However, in the production, melon faces many problems. One of them is Powdery Mildew attack. Consequently, it is needed to create a new cultivar that has gene against the attack. Tacapa Green Black (GB) was one of cultivars developed by Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada. Tacapa GB was derived between PI 371795 and Act3 434 with a test cross Act3 434. The goal of this research was detection of a powdery mildew resistance gene. It was conducted using Sequence Characterized Amplified Regions (SCARs) analysis. The comparator cultivars used in this research were Tacapa Silver, Action 434, and Aramis. The result showed that Tacapa GB had a resistance gene of powdery mildew. It was proven with DNA band at 1058 base pair (bp). Based on this result, it could be concluded that Tacapa GB was excellent melon because of an ability in facing Powdery Mildew problems naturally. Furthermore, because of the presence of that gene, it could reduce the using of pesticide which was commonly used by Farmers to handle fungi attack.

Highlights

  • Melon (Cucumis melo L.) is one of excellent horticultural commodities in Indonesia

  • Based on the data given from Badan Pusat Statistika (BPS), the melon production reached 85,161 ton in 2010, 103,840 ton in 2011, 125,474 ton in 2012, and 125,207 ton in 2013 [2]

  • Tacapa Green Black (GB) is one of the cultivar developed by Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada, Indonesia

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Summary

Introduction

Melon (Cucumis melo L.) is one of excellent horticultural commodities in Indonesia. The seed’s demand grows considerably in every year which is marked by the growth of melon production in Indonesia. Based on the data given from Badan Pusat Statistika (BPS), the melon production reached 85,161 ton in 2010, 103,840 ton in 2011, 125,474 ton in 2012, and 125,207 ton in 2013 [2]. Tacapa Green Black (GB) is one of the cultivar developed by Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada, Indonesia. The previous experiments indicated that PI 371795 had gene resistence of Powdery Mildew [3]. It forecasts that Tacapa GB inherits this superior character

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