Abstract

Powdery mildew is one of the diseases caused by fungal infections that can reduce the production of melon fruit worldwide including in Indonesia. A powdery mildew-resistant cultivar of melon is needed to increase melon yield crops. This study aimed to detect resistance gene linked to powdery mildew using a sequence characterized amplified region (SCAR) markers. The melon cultivar Meloni was used in this study. SL-3, PI 371795, and Aramis cultivar were used to compare. Amplification of the marker was performed employing a pair of primers. The result showed that Meloni had a powdery mildew resistance gene by the presence of a DNA target band at 1058 base pair (bp). Based on this result, it could be concluded that Meloni was an excellent melon cultivar because of its ability to overcome the powdery mildew infections naturally. SCAR markers have been used for various purposes, especially to detect resistance genes to plant diseases. The present study had provided information for plant breeders about Meloni as the new melon cultivar that was genetically resistant against powdery mildew infections. Furthermore, Meloni could be proposed as an alternative to native Indonesian superior melon seeds.

Highlights

  • Wrinkled while the stem becomes chlorotic and Indonesia is a tropical country with abundant essential resources, and one of them is Melon (Cucumis melo L.)

  • Powdery mildew is a plant disease caused by fungal infections, and it is a big problem for farmers

  • In addition to using fungicides, powdery mildew can be managed by plant breeding with crossing melon cultivars that are resistant to this disease

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Summary

Introduction

Wrinkled while the stem becomes chlorotic and Indonesia is a tropical country with abundant essential resources, and one of them is Melon (Cucumis melo L.). Powdery mildew is a plant disease caused by fungal infections, and it is a big problem for farmers. The leaf becomes brown dies (Daryono & Maryono, 2017) Infection by these fungi results in reduced melon resistance that can lower the harvest quality, sugar content and aroma of the fruit. Excessive use of fungicides can affect the environment while continual use can cause fungi to become more resistant (Candido et al, 2013). In addition to using fungicides, powdery mildew can be managed by plant breeding with crossing melon cultivars that are resistant to this disease. PI 371795 has superior properties against powdery mildew infections (Aristya & Daryono, 2013). Markers from RAPD to detect powdery mildew resistance gen (Pm-I) in melon accurately. SCAR analysis in diverse melons detected a single 1058 bp linked to Pm-I in resistant melon

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