Abstract
ABSTRACTIllumina sequencing of RNA from a cassava cutting from northern Malawi produced a genome of Ugandan cassava brown streak virus (UCBSV-MW-NB7_2013). Sequence comparisons revealed stronger similarity to an isolate from nearby Tanzania (93.4% pairwise nucleotide identity) than to those previously reported from Malawi (86.9 to 87.0%).
Highlights
Illumina sequencing of RNA from a cassava cutting from northern Malawi produced a genome of Ugandan cassava brown streak virus (UCBSV-MW-NB7_2013)
Genomea.asm.org 1 revealed unexpected diversity in the 5= untranslated region (UTR) [6], and further sequencing of UCBSV isolates could reveal that the 5= terminus is more variable than previously thought
Sequence comparisons revealed stronger similarity to an isolate from nearby Tanzania (93.4% pairwise nucleotide identity) than to those previously reported from Malawi (86.9 to 87.0%)
Summary
Illumina sequencing of RNA from a cassava cutting from northern Malawi produced a genome of Ugandan cassava brown streak virus (UCBSV-MW-NB7_2013). Pairwise nucleotide sequence similarity of UCBSV-MW-NB7_2013 with UCBSV genome sequences showed 87.9 to 93.4% nucleotide identities, with the highest identity to the sequence of the UCBSV MLB3 isolate (FJ039520) from Tanzania. The amino acid sequence of both polyproteins was 95.8% identical.
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