De Novo Assembly of Candida sojae and Candida boidinii Genomes, Unexplored Xylose-Consuming Yeasts with Potential for Renewable Biochemical Production.

Guilherme Borelli,Paulo José Pereira Lima Teixeira,Gonçalo Amarante Guimarães Pereira,Juliana José,Leandro Vieira dos Santos

doi:10.1128/genomea.01551-15

Guilherme Borelli, Paulo José Pereira Lima Teixeira + Show 3 more

Open Access

https://doi.org/10.1128/genomea.01551-15

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Abstract

Candida boidinii and Candida sojae yeasts were isolated from energy cane bagasse and plague-insects. Both have fast xylose uptake rate and produce great amounts of xylitol, which are interesting features for food and 2G ethanol industries. Because they lack published genomes, we have sequenced and assembled them, offering new possibilities for gene prospection.

Highlights

Candida boidinii and Candida sojae are both xyloseconsuming yeasts from the Saccharomycetales order of Ascomycota
Samples were sent to the University of North Carolina at the High-Throughput Sequencing Facility (HTSF) for genome sequencing using an Illumina HiSeq2000, which produced a library with insert sizes around 400 nucleotides and near to 116 million paired reads for C. boidinii and 109 million for C. sojae, with 100 nt each
We proceeded with the C. sojae assembly using the dipSPAdes module, which resulted in a high-quality assembly for a consensus haploid genome

Summary

Introduction

Candida boidinii and Candida sojae are both xyloseconsuming yeasts from the Saccharomycetales order of Ascomycota. C. boidinii was found as a methylotrophic yeast (1), while C. sojae lacks further studies of its metabolic pathways. From the yeast strains we have isolated, these Candida isolates were shown as the best strains in xylose consumption (C. boidinii and C. sojae) and in xylitol production (C. sojae; unpublished results), a sugar-alcohol interesting for food industry as a sweetener (4).

Results

Conclusion