Correlations between the mannose-binding lectin (MBL) gene polymorphisms and the susceptibility to tuberculosis in Tibetan population in Qinghai province

Abstract

Objective To investigate the correlations between the mannose-binding lectin (MBL) gene polymorphisms and the susceptibility to tuberculosis in Tibetan population in Qinghai province. Methods Ninety-nine Tibetan patients with pulmonary tuberculosis in Qinghai province were recruited as the experimental group and eighty-nine healthy Tibetan subjects were recruited as the control group. The genotypes of 6 single nucleotide polymorphisms (SNPs) in MBL gene including the start zone-550-H/L (rs11003125), + 4P/Q (rs7095891) and -221X/Y (rs7096206) as well as the exon 1 codon 52 (allele D) (rs5030737), codon 54 (allele B) (rs1800450) and codon 57 (allele C) (rs1800451) were analyzed by using TaqMan probe real-time quantitative polymerase chain reaction (TaqMan probe method). SPSS20.0 software was used to comparatively analyze the differences in the genotypes and haplotypes of MBL gene between the two groups. Results The allele frequency in rs5030737 was not in accordance with Hardy-Weinberg equilibrium. Significant differences in the genotypes between the two groups were observed in rs1800450, rs7095891 and rs7096206 (P 0.05). Conclusion The MBL gene polymorphisms in rs1800450, rs7096206 and rs7095891 sites were associated with the susceptibility to tuberculosis in Tibetan population in Qinghai province. Key words: Tibetan; Tuberculosis; Mannose-binding lectin gene polymorphisms