Conditions for surface cultivation on liquid nutrient media of the producer strain of microbiopreparation M-24 Penicillium sp., the antagonist of the sunflower Phoma rot pathogen

Abstract

In recent years (2019-2021), research has been carried out at the biomethod laboratory of the V.S. Pustovoit All-Russian Research Institute of Oil Crops on the development of environmentally safe microbiopreparations of a multifunctional type of action with a prolonged shelf life against the pathogen of sunflower Phoma rot caused by the fungus Phoma macdonaldii Boerema, a teleomorph is Plenodomus lindquistii Gruyter, Aveskamp & Verkley. To develop the elements of the technological regulations for the production of a microbiopreparations in the preparative form of a “wettable powder” when growing the producer strain M-24 Penicillium sp. on the liquid nutrient media, there were determined the following optimal conditions for cultivating the strain: temperature is 25 °C, pH is 6; to use starch as a source of carbon nutrition is advisable; the optimal source of nitrogen nutrition is corn extract.