Биологические особенности и условия поверхностного культивирования штаммапродуцента микробиопрепарата 11-3 Bacillus sp. – антагониста возбудителей фузариоза льна масличного

Abstract

To develop a technological regimen of production of microbiopreparation in a preparative form ‘wettable powder’ (WP) we studied biological features and conditions of surface cultivation of the strainproducer 11-3 Bacillus sp. – an antagonist of fusariosis pathogen infecting oil flax. Cultural qualities of the strain-producer were studied in three agarized mediums. The optimal conditions for cultivation of the strain-producer on liquid nutrient mediums are estimated: temperature – 30–35 оС, рН – 8–10. As a source of carbon nutrition, glycerin and molasses can be used. Yeastrel and corn-steep concentrate are the optimal sources of nitrogen nutrition; the Tylon’s medium is optimal compound liquid nutrient medium for cultivation of the perspective bacterial strain 11-3 Bacillus sp. Optimal period of the surface cultivation of the bacterial strain 11-3 Bacillus sp. on the liquid nutrient Tylon’s medium was ten days, and a volume of sowing culture to nutrient medium – 2.0%.