Abstract
We studied the cultural and physiological characteristics of the producer strain 11-1 Bacillus sp. – the antagonist of sunflower Phoma rot pathogen to develop the technological regulations for the production of a microbiological preparation in a «wettable powder» form. We studied the cultural characteristics of the producer strain on three agar media: potato sucrose agar (PSA), Czapek’s agar and Tylon-3; the characteristics varied significantly depending on the nutrient medium. The maximum diameter of colonies on the tenth day of incubation developed on the Tylon-3 medium – 66×99 mm. We established the optimal conditions for the cultivation of the strain on liquid nutrient media: temperature – 30–35 °С, medium pH from 6 to 10. We found that molasses is an optimal source of carbon nutrition, while peptone is the most favorable source of nitrogen nutrition. We established that the Tylon-3 medium is the optimal complex liquid nutrient medium for the cultivation of the bacterial strain 11-1 Bacillus sp.
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