Abstract
ABSTRACTBrucellosis is a zoonotic disease that affects both humans and animals. Its distribution is global, concentrated in the Mediterranean area, India, Central Asia, and Latin America. Here, we present a complete genome assembly of 10 Brucella abortus strains isolated from water buffaloes farmed in the Campania region of Italy.
Highlights
Brucellosis is one of the most widespread bacterial zoonotic diseases in the world, affecting both humans and domestic and wild animals (1)
Sequencing reads were checked for quality using FastQC, and low-quality sequences were removed using PRINSEQ lite
We identified all coding genes and genes coding for proteins involved in virulence mechanisms
Summary
Brucellosis is one of the most widespread bacterial zoonotic diseases in the world, affecting both humans and domestic and wild animals (1). Strains were inoculated onto Brucella medium base (Oxoid) and incubated at 37°C in 5 to 10% CO2. DNA was purified with the QIAamp DNA minikit (Qiagen), as described by the manufacturer.
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