Abstract
ABSTRACTWe present here the full-length genome sequences of two hepatitis E virus genotype 3 (HEV-3) isolates from a human stool sample from a patient with acute hepatitis and a raw sausage containing pig liver. Sequence analysis implies that Swiss HEV isolates may form a novel subgroup of HEV-3 viruses.
Highlights
Hepatitis E virus (HEV) is a major cause of acute hepatitis worldwide
To prepare the RNA samples for next-generation sequencing (NGS), sequence-independent singleprimer amplification was performed (6), and the purified amplicons were used for the construction of sequencing libraries using the NEBNext Ultra II library preparation kit (BioConcept, Allschwil, Switzerland)
A paired-end NGS run of 2 ϫ 150 nucleotide read length was performed at the Functional Genomic Center Zurich using the Illumina NextSeq 500 machine
Summary
Hepatitis E virus (HEV) is a major cause of acute hepatitis worldwide. It is a small nonenveloped or quasienveloped virus (1) with a positive-sense single-stranded RNA genome of approximately 7.2 kb and belongs to the family Hepeviridae. We present the full-genome sequences of two HEV isolates from a human and a food sample. The stool sample originated in a 78-year-old male hospitalized in October 2016 in Lugano (Canton Ticino, southern Switzerland) with acute hepatitis and was collected within 10 days from symptom onset. The food sample was a traditional raw dry-cured pork sausage containing pork liver (“mortadella di fegato crudo”), sold by a local butcher shop.
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