Abstract
ABSTRACTThe genomes of Lactobacillus curvatus KG6, L. curvatus MRS6, and Lactobacillus sakei FAM18311 were sequenced and assembled using PacBio single-molecule real-time (SMRT) technology. The strains were isolated from Swiss fermented meat products. Circular chromosomes were of 1.98 Mbp (KG6), 2.11 Mbp (MRS6), and 1.95 Mbp (FAM18311), with a G+C content of 41.3 to 42.0%.
Highlights
The genomes of Lactobacillus curvatus KG6, L. curvatus MRS6, and Lactobacillus sakei FAM18311 were sequenced and assembled using PacBio single-molecule real-time (SMRT) technology
An overnight culture was used to propagate the strains under anaerobic conditions at 37°C in MRS broth (Biolife) for DNA isolation
One single-molecule real-time (SMRT) cell per strain was used for sequencing of the genomic DNA on a PacBio RSII system (Pacific Biosciences, Menlo Park, CA, USA) at the Functional Genomics Center Zurich (Zurich, Switzerland)
Summary
The genomes of Lactobacillus curvatus KG6, L. curvatus MRS6, and Lactobacillus sakei FAM18311 were sequenced and assembled using PacBio single-molecule real-time (SMRT) technology. An overnight culture was used to propagate the strains under anaerobic conditions at 37°C in MRS broth (Biolife) for DNA isolation. The Wizard genomic DNA purification kit (Promega, Madison, WI, USA) was used for DNA isolation, including modifications as previously described [7]. One single-molecule real-time (SMRT) cell per strain was used for sequencing of the genomic DNA on a PacBio RSII system (Pacific Biosciences, Menlo Park, CA, USA) at the Functional Genomics Center Zurich (Zurich, Switzerland). Genome assembly of KG6 and MRS6 was performed using the SMRT Analysis system SMRT Portal (version 2.3.0.140936.p1.142411; Pacific Biosciences).
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