Abstract
ABSTRACTWe report four full-genome sequences of bovine coronavirus (BCoV) isolates from dairy calves in Pennsylvania obtained in 2016 and 2017. BCoV is a pathogen of great importance to cattle health, and this is the first report of full-genome sequences of BCoV from PA cattle.
Highlights
Bovine coronavirus (BCoV), a member of the Betacoronavirus genus of the Coronaviridae family, is an enveloped virus with an approximately 31-kb single-stranded positive-sense RNA genome
Following alignment of full-genome nucleotide sequences by the MUSCLE algorithm implemented in the MEGA7 package [5, 6], phylogenetic analysis indicated that the four isolates were closely related to one another and shared 99% coverage and 99% identity with their closest relative, sable antelope coronavirus US/OH1/2003 (GenBank accession number EF424621)
The ORF1ab, HE, S, NS5, E, M, and N genes of the four sequences were subjected to BLASTN analysis [7, 8]
Summary
Bovine coronavirus (BCoV), a member of the Betacoronavirus genus of the Coronaviridae family, is an enveloped virus with an approximately 31-kb single-stranded positive-sense RNA genome. The four sequences generated contained 31,016 to 31,032 nucleotides, with 99.36% nucleotide identity between isolates. Following alignment of full-genome nucleotide sequences by the MUSCLE algorithm implemented in the MEGA7 package [5, 6], phylogenetic analysis indicated that the four isolates were closely related to one another and shared 99% coverage and 99% identity with their closest relative, sable antelope coronavirus US/OH1/2003 (GenBank accession number EF424621).
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