Abstract
ABSTRACTWe report here the complete genome sequence of a West Nile virus (WNV) strain from the first laboratory-confirmed human case of neuroinvasive WNV infection in Cyprus. Phylogenetic analysis showed that this WNV strain grouped clearly into genetic lineage 1, clade 1a, cluster 2.
Highlights
West Nile virus (WNV) is a neurotropic mosquito-borne pathogen belonging to the Flavivirus genus within the Flaviviridae family
The sequenced genome comprises 10,982 nucleotides with a polyprotein of 3,434 amino acids being encoded within a single open reading frame
Phylogenetic analysis showed that the WNV strain from Cyprus grouped clearly into genetic lineage 1, clade 1a, cluster 2, and had the highest nucleotide identity with a strain isolated recently from a dromedary camel in the United Arab Emirates in 2015 [6, 7]
Summary
West Nile virus (WNV) is a neurotropic mosquito-borne pathogen belonging to the Flavivirus genus within the Flaviviridae family. It was shown that whole-transcriptome amplification techniques, which are based on multiple-displacement amplification, can be successfully used for amplification of RNA virus genomes [4]. Because of the limited amount of viral genomic material available, a similar approach was employed here using the Quantitect wholetranscriptome kit (Qiagen) to increase the amount of cDNA necessary for sequencing the complete genome.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.