Abstract

ABSTRACTWe report here the complete genome sequence of a West Nile virus (WNV) strain from the first laboratory-confirmed human case of neuroinvasive WNV infection in Cyprus. Phylogenetic analysis showed that this WNV strain grouped clearly into genetic lineage 1, clade 1a, cluster 2.

Highlights

  • West Nile virus (WNV) is a neurotropic mosquito-borne pathogen belonging to the Flavivirus genus within the Flaviviridae family

  • The sequenced genome comprises 10,982 nucleotides with a polyprotein of 3,434 amino acids being encoded within a single open reading frame

  • Phylogenetic analysis showed that the WNV strain from Cyprus grouped clearly into genetic lineage 1, clade 1a, cluster 2, and had the highest nucleotide identity with a strain isolated recently from a dromedary camel in the United Arab Emirates in 2015 [6, 7]

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Summary

Introduction

West Nile virus (WNV) is a neurotropic mosquito-borne pathogen belonging to the Flavivirus genus within the Flaviviridae family. It was shown that whole-transcriptome amplification techniques, which are based on multiple-displacement amplification, can be successfully used for amplification of RNA virus genomes [4]. Because of the limited amount of viral genomic material available, a similar approach was employed here using the Quantitect wholetranscriptome kit (Qiagen) to increase the amount of cDNA necessary for sequencing the complete genome.

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