Abstract
Streptomyces phage Nanodon is a temperate double-stranded DNA Siphoviridae belonging to cluster BD1. It was isolated from soil collected in Kilauea, HI, using Streptomyces griseus subsp. griseus as a host.
Highlights
Streptomyces spp. are Gram-positive, filamentous, and sporeforming members of the Actinomycetaceae family, from which two-thirds of antibiotics have been derived [1]
We present the genome of the double-stranded DNA bacteriophage Nanodon, a Siphoviridae isolated from volcanic soil collected in Kilauea, HI (22°10=28.6ЉN 159°26=08.3ЉW), which was harvested and investigated using Streptomyces griseus subsp. griseus (ATCC 10137) (S. griseus) as a host by University of Maryland, Baltimore County (UMBC) undergraduate researchers, as described previously [2]
Nanodon produced variably sized clear plaques when incubated for 48 h on lawns of S. griseus grown on supplemented nutrient agar at 30°C [3]
Summary
Streptomyces spp. are Gram-positive, filamentous, and sporeforming members of the Actinomycetaceae family, from which two-thirds of antibiotics have been derived [1]. We present the genome of the double-stranded DNA bacteriophage Nanodon, a Siphoviridae isolated from volcanic soil collected in Kilauea, HI (22°10=28.6ЉN 159°26=08.3ЉW), which was harvested and investigated using Streptomyces griseus subsp. Griseus (ATCC 10137) (S. griseus) as a host by University of Maryland, Baltimore County (UMBC) undergraduate researchers, as described previously [2].
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