Abstract
ABSTRACTStaphylococcus aureus JH4899, a community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) isolate collected from a patient with systematically disseminated infection, is classified as sequence type 8 and carries the staphylococcal cassette chromosome mec type IVl (SCCmecIVl). It produces TSST-1, SEC, a newly discovered enterotoxin (SE1), and epidermal cell differentiation inhibitor A (EDIN-A). Here, we present the complete genome sequence of the chromosome and a plasmid harboring the se1 and ednA genes.
Highlights
Staphylococcus aureus JH4899, a community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) isolate collected from a patient with systematically disseminated infection, is classified as sequence type 8 and carries the staphylococcal cassette chromosome mec type IVl (SCCmecIVl)
We previously reported a patient with systematically disseminated CA-MRSA/J infection and described the genotypic analysis (3)
The genomic DNA was isolated from JH4899 grown on tryptic soy broth (TSB) at 37°C using the QIAamp DNA minikit (Qiagen, USA) with the addition of lysostaphin (10 g/mL) and incubation at 37°C for 1 h
Summary
Staphylococcus aureus JH4899, a community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) isolate collected from a patient with systematically disseminated infection, is classified as sequence type 8 and carries the staphylococcal cassette chromosome mec type IVl (SCCmecIVl). We previously reported a patient with systematically disseminated CA-MRSA/J infection and described the genotypic analysis (3). We report the complete genome sequence of CA-MRSA/J JH4899, which carries the staphylococcal cassette chromosome mec type IVl (SCCmecIVl) isolated in 2012 from the expectorated sputum of the patient.
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