Abstract

ABSTRACTWe report the closed and annotated genome sequence of Sulfuriferula sp. strain AH1. Strain AH1 has a 2,877,007-bp chromosome that includes a partial Sox system for inorganic sulfur oxidation and a complete nitrogen fixation pathway. It also has a single 39,138-bp plasmid with genes for arsenic and mercury resistance.

Highlights

  • We report the closed and annotated genome sequence of Sulfuriferula sp. strain AH1

  • Strain AH1 is a member of the genus Sulfuriferula but is Ͻ97% similar by 16S rRNA gene sequence to the three described Sulfuriferula species [10,11,12]

  • Cells of strain AH1 were collected from batch cultures onto 0.2-␮m-pore filters, and genomic DNA was isolated from the filters using the PowerWater DNA isolation kit (Mo Bio Laboratories, Inc.)

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Summary

Introduction

We report the closed and annotated genome sequence of Sulfuriferula sp. strain AH1. Strain AH1 has a 2,877,007-bp chromosome that includes a partial Sox system for inorganic sulfur oxidation and a complete nitrogen fixation pathway. Strain AH1 is a member of the genus Sulfuriferula but is Ͻ97% similar by 16S rRNA gene sequence to the three described Sulfuriferula species [10,11,12]. Cells of strain AH1 were collected from batch cultures onto 0.2-␮m-pore filters, and genomic DNA (gDNA) was isolated from the filters using the PowerWater DNA isolation kit (Mo Bio Laboratories, Inc.). Twenty micrograms of high-molecular-weight gDNA was used for long-read sequencing on a Pacific Biosciences RSII platform.

Results
Conclusion

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