Complete Genome Sequence of Streptococcus pneumoniae Virulent Phage MS1.

Witold Kot,Mourad Sabri,Sylvain Moineau,Hélène Gingras,Denise M Tremblay,Marc Ouellette

doi:10.1128/genomea.00333-17

Abstract

ABSTRACTThe lytic Streptococcus pneumoniae phage MS1 was isolated from a throat swab of a patient with symptoms of upper respiratory tract infection. The genome of this siphophage has 56,075 bp, 42.3% G+C content, and 77 open reading frames, including queuosine biosynthesis genes. Phage MS1 is related to pneumococcal phage Dp-1.

Highlights

The lytic Streptococcus pneumoniae phage MS1 was isolated from a throat swab of a patient with symptoms of upper respiratory tract infection
A new virulent pneumococcal phage was isolated from a swab sample collected at the Centre Hospitalier de l’Université Laval (Quebec City)
The swab sample was eluted, filtered (0.45 ␮m), and propagated on S. pneumoniae strain R6 using brain heart infusion (BHI) broth supplemented with 5 ng/mL choline chloride (BHI-fc) and incubated overnight at 37°C [2]

Summary

Introduction

The lytic Streptococcus pneumoniae phage MS1 was isolated from a throat swab of a patient with symptoms of upper respiratory tract infection. A new virulent pneumococcal phage was isolated from a swab sample collected at the Centre Hospitalier de l’Université Laval (Quebec City). Several phage plaques were obtained and based on the DNA restriction profile of their genome, only one distinct phage was isolated, purified, and designated MS1. Genomic DNA of phage MS1 was isolated using a Lambda maxi kit (Qiagen) from a lysate. Genome sequencing was performed on a 454 FLX instrument (IBIS, Université Laval).

Results

Conclusion