Complete Genome Sequence of Salmonella enterica Serovar Typhimurium Strain YU15 (Sequence Type 19) Harboring the Salmonella Genomic Island 1 and Virulence Plasmid pSTV.

Claudia Silva,Pablo Vinuesa,José L Puente,Edmundo Calva,Mussaret B Zaidi

doi:10.1128/genomea.00252-16

Abstract

The complete genome of Salmonella enterica subsp. enterica serovar Typhimurium sequence type 19 (ST19) strain YU15, isolated in Yucatán, Mexico, from a human baby stool culture, was determined using PacBio technology. The chromosome contains five intact prophages and the Salmonella genomic island 1 (SGI1). This strain carries the Salmonella virulence plasmid pSTV.

Highlights

The complete genome of Salmonella enterica subsp. enterica serovar Typhimurium sequence type 19 (ST19) strain YU15, isolated in Yucatán, Mexico, from a human baby stool culture, was determined using PacBio technology
We present the complete genome sequence of Salmonella enterica subsp. enterica serovar Typhimurium strain YU15, which was isolated in 2000 in Yucatán, Mexico, from a stool culture from a 3-month-old child with diarrhea, as part of an epidemiological surveillance program in which it was referred as YUHS 00-228 [1, 2]
Genomic DNA was extracted by standard protocols [4] and sheared into ~10- to 20-kb fragments for PacBio library preparation and P6-C4 sequencing on one single-molecule real-time (SMRT) cell

Summary

Introduction

The complete genome of Salmonella enterica subsp. enterica serovar Typhimurium sequence type 19 (ST19) strain YU15, isolated in Yucatán, Mexico, from a human baby stool culture, was determined using PacBio technology. We present the complete genome sequence of Salmonella enterica subsp. Enterica serovar Typhimurium strain YU15, which was isolated in 2000 in Yucatán, Mexico, from a stool culture from a 3-month-old child with diarrhea, as part of an epidemiological surveillance program in which it was referred as YUHS 00-228 [1, 2]. Genomic DNA was extracted by standard protocols [4] and sheared into ~10- to 20-kb fragments for PacBio library preparation and P6-C4 sequencing on one single-molecule real-time (SMRT) cell.

Results

Conclusion