Abstract
Here we report the complete genomic sequence of the SBM2 strain (VSBV-SBM2) of the sacbrood virus (SBV) that was isolated from the Asian honeybee (Apis cerana) in Northern Vietnam. The entire sequence excluding the 3′ poly(A) tail is 8,834 nucleotides in length and contains a single large open reading frame (ORF) of 8,580 nucleotides (position 178 to 8757), encoding 2,859 amino acids. VSBV-SBM2 shared 90 to 93% nucleotide identity and 95 to 96% amino acid homology to six complete genomes of SBV currently available in GenBank (two from China, three from Korea, and one from the United Kingdom). A hypervariable domain (amino acid [aa] position 712 to 729) and a conserved motif (position 2124 to 2143) in the precursor polypeptide of all seven SBVs are also described.
Highlights
We report the complete genomic sequence of the SBM2 strain (VSBV-SBM2) of the sacbrood virus (SBV) that was isolated from the Asian honeybee (Apis cerana) in Northern Vietnam
The sacbrood virus (SBV) primarily infects broods of both common European (Apis mellifera) and Asian (Apis cerana) honeybee species, causing larva death due to failure of pupation that leads to the formation of a sac-like shape
Ten overlapping fragments were obtained by PCR using five pairs of oligonucleotide primers designed based on the alignment of the full-length genome sequences of the available SBV strains listed above
Summary
We report the complete genomic sequence of the SBM2 strain (VSBV-SBM2) of the sacbrood virus (SBV) that was isolated from the Asian honeybee (Apis cerana) in Northern Vietnam. The sacbrood virus (SBV) primarily infects broods of both common European (Apis mellifera) and Asian (Apis cerana) honeybee species, causing larva death due to failure of pupation that leads to the formation of a sac-like shape. Six complete genome sequences of SBVs are available: strain Rothamstead (GenBank: AF092924) from the United Kingdom; AmSBV-Kor19 (JQ390592), AmSBV-Kor21 (JQ390591), and AcSBV-Kor (HQ322114) from South Korea; and CSBV-LN (HM237361) and CSBV-GZ (AF469603) from China [1,2,3].
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