Abstract
ABSTRACTVibrio vulnificus, a ubiquitous inhabitant of coastal marine environments, has been isolated from a variety of sources. It is an opportunistic pathogen of both marine animals and humans. Here, the genome sequence of V. vulnificus Env1, an environmental isolate resistant to predation by the ciliate Tetrahymena pyriformis, is reported.
Highlights
Vibrio vulnificus, a ubiquitous inhabitant of coastal marine environments, has been isolated from a variety of sources
The sequences were submitted to the Rapid Annotations using Subsystems Technology (RAST) (6) as well as the NCBI prokaryotic genome annotation pipeline (7) servers, and results show that there are a total of 4,378 coding sequences (CDSs) and 157 RNAs predicted
The majority of the CDSs were related to functions in subsystems, including amino acids and derivatives (515 genes), carbohydrates (456), protein metabolism (310), and the synthesis of cofactors, vitamins, prosthetic groups, and pigments (303 genes)
Summary
A ubiquitous inhabitant of coastal marine environments, has been isolated from a variety of sources. Genomic DNA was extracted according to the manufacturer’s instructions using the Qiagen DNeasy genomic DNA prep kit for Gram-negative bacterial cultures, and genomic DNA libraries (15 to 20 Kb) were generated by the Ramaciotti Centre (University of New South Wales, Sydney, Australia), according to the manufacturer’s instructions. The complete genome sequence of V. vulnificus Env[1] was generated using the PacBio RS platform with single-molecule real-time (SMRT) sequencing with 140ϫ coverage.
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