Abstract

ABSTRACTMycobacterium stephanolepidis is a rapid-growing nonpigmented species isolated from marine teleost fish (Stephanolepis cirrhifer) and is closely related to Mycobacterium chelonae. Here, we report the complete sequence of its genome, comprising a 4.9-Mb chromosome. The sequence represents essential data for future phylogenetic and comparative genome studies of this fish pathogen.

Highlights

  • Mycobacterium stephanolepidis is a rapid-growing nonpigmented species isolated from marine teleost fish (Stephanolepis cirrhifer) and is closely related to Mycobacterium chelonae

  • The chromosome contains 4,981 predicted protein-coding sequences (CDSs), which is similar to the number of CDSs in M. chelonae strain CCUG 47445 (4,921 CDSs)

  • This complete genome sequence has been deposited at DDBJ/ENA/GenBank under the GenBank accession no

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Summary

Introduction

Mycobacterium stephanolepidis is a rapid-growing nonpigmented species isolated from marine teleost fish (Stephanolepis cirrhifer) and is closely related to Mycobacterium chelonae. The strain was grown on Middlebrook 7H10 agar medium. DNA was purified with the QIAamp DNA minikit (Qiagen, Hilden, Germany) and NucleoSpin plant II kit (MachereyNagel, Düren, Germany).

Results
Conclusion

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