Abstract
The murine pneumotropic virus genome encoded by the pKV(37-1) clone was sequenced to completion. The regulatory region harbored a mutation not previously reported. The protein coding regions (large and small T antigens, viral proteins 1 to 3) showed multiple regions of high amino acid identity to the human, simian, and bovine polyomaviruses.
Highlights
The murine pneumotropic virus genome encoded by the pKV[37-1] clone was sequenced to completion
PKV[37-1], encoding the entire Murine pneumotropic virus (MPtV) genome inserted into the PstI site of pBR322, obtained from the American Type Culture Collection (ATCC) [7], as the plasmid sequence was not previously reported
We found the noncoding regulatory region, which contains the origin of viral DNA replication as well as all transcriptional regulatory sequences, to be most consistent with one of the variants ([9]; AJ517509), which contained a 220-bp insertion at nucleotide 142 of a cellular transposable element in combination with a small deletion
Summary
The murine pneumotropic virus genome encoded by the pKV[37-1] clone was sequenced to completion. Murine pneumotropic virus (MPtV), previously Kilham polyomavirus, was first isolated/described over 50 years ago [1, 2]. The MPtV complete genome was originally sequenced (GenBank accession number NC_001505) to elucidate the genomic organization and relationship to Polyomaviridae [5] and more recently sequenced (KT987216 to KT987218) to explore the diversity of MPtV in wild mice [6].
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