Abstract
RB43-related bacteriophages have a specific genome type that clearly distinguishes them from other T4-like viruses. Here, we present the complete genome sequence of a new virulent phage, Lw1, isolated as an Escherichia coli BL21(DE3) contaminant. Lw1 shares an RB43-like genome organization, but it does not contain putative AP2-domain endonuclease genes.
Highlights
RB43-related bacteriophages have a specific genome type that clearly distinguishes them from other T4-like viruses
We report the annotation of the complete genome sequence of phage Lw1, which shares an RB43like genome arrangement but does not contain putative AP2domain genes
Lw1 was isolated as a contaminating phage from lysed cells of Escherichia coli BL21(DE3)
Summary
RB43-related bacteriophages have a specific genome type that clearly distinguishes them from other T4-like viruses. The taxonomy of T4-like bacteriophages is questionable and does not reflect existing relationships between the viruses belonging to this group. The isolation and sequencing of additional members of already-existing subgroups of T4-like phages are essential for improvement of the current classifications.
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