Abstract
ABSTRACTThe sequence type 131 (ST131)-H30 clone is responsible for a significant proportion of multidrug-resistant extraintestinal Escherichia coli infections. Recently, the C1-M27 clade of ST131-H30, associated with blaCTX-M-27, has emerged. The complete genome sequence of E. coli isolate 81009 belonging to this clone, previously used during the development of ST131-specific monoclonal antibodies, is reported here.
Highlights
The sequence type 131 (ST131)-H30 clone is responsible for a significant proportion of multidrug-resistant extraintestinal Escherichia coli infections
Hierarchical Genome Assembly Process 4 (HGAP4) [5] was used for de novo assembly of the PacBio reads, and Illumina short reads were aligned to the de novo assembled genome with the Burrows-Wheeler Alignment tool (BWA) [6], followed by indel realignment and base quality recalibration with Genome Analysis Toolkit (GATK) [7]
Protein-coding genes were called with Prodigal within the Prokka annotation pipeline [8]
Summary
The sequence type 131 (ST131)-H30 clone is responsible for a significant proportion of multidrug-resistant extraintestinal Escherichia coli infections. Protein-coding genes were called with Prodigal within the Prokka annotation pipeline [8]. Trusted protein sequences for the annotation step were obtained from E. coli ST131 EC958 [9]. Additional coding and noncoding RNA genes, as well as signal peptides, were predicted by RNAmmer, ARAGORN, SignalP, and Infernal within the Prokka annotation pipeline.
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