Abstract
ABSTRACTThe lytic Escherichia coli phage Ebrios was isolated from a water sample collected in Ebrie Lagoon on the Adiopodoumé River in Abidjan (Republic of Côte d’Ivoire, West Africa). The linear genome of this Podoviridae family member contains 39,752 bp, has a G+C content of 52.9%, is composed of 53 open reading frames, and is related to the Stenotrophomonas maltophilia phage IME15.
Highlights
The lytic Escherichia coli phage Ebrios was isolated from a water sample collected in Ebrie Lagoon on the Adiopodoumé River in Abidjan (Republic of Côte d’Ivoire, West Africa)
The genomic DNA of phage Ebrios was extracted from a high-titer lysate (109 PFU · mlϪ1) using a plasmid maxikit (Qiagen) with modifications (5)
The Ebrios genome is highly related to the genomes of Stenotrophomonas maltophilia phage IME15 (9) and E. coli podophage T7
Summary
The lytic Escherichia coli phage Ebrios was isolated from a water sample collected in Ebrie Lagoon on the Adiopodoumé River in Abidjan (Republic of Côte d’Ivoire, West Africa). The genomic DNA of phage Ebrios was extracted from a high-titer lysate (109 PFU · mlϪ1) using a plasmid maxikit (Qiagen) with modifications (5). The sequencing library was prepared with the Nextera XT DNA library preparation kit (Illumina), according to the manufacturer’s instructions, and sequenced using a MiSeq reagent kit v2 (500 cycles; Illumina) on a MiSeq instrument. De novo assembly was performed with the Ray assembler version 2.2.0 (6).
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