Abstract
ABSTRACTA temperate phage, SEN8, having a broad activity against pathogenic Salmonella serovars, was isolated from Salmonella enterica subsp. salamae strain Sen8. The complete genome sequence of phage SEN8 was determined (35,203 bp) and showed relatedness to P2-like phages (Salmonella phages Fels-2 and RE-2010).
Highlights
Infections of Salmonella spp. are an important public health problem
Phage particles were multiplied from a single plaque on the S. enterica subsp. salamae indicator 356/03 (Sen9) and isolated using a CsCl gradient
Based on BLASTn analysis, the genome sequence of the SEN8 phage shared the greatest similarity (i.e., 77% and 68%) with Salmonella phages Fels-2 (NC_010463) and RE-2010 (NC_019488), respectively, both of which were P2-like phages
Summary
Infections of Salmonella spp. are an important public health problem. While S. enterica subsp. enterica causes about 99% of human salmonelloses [1], the remaining sporadic infections are caused by Salmonella strains from subspecies salamae, arizonae, diarizonae, and houtenae [2]. Phage particles were multiplied from a single plaque on the S. enterica subsp. Salamae indicator 356/03 (Sen9) and isolated using a CsCl gradient.
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