Abstract
The Bacillus cereus emetic pathotype is responsible for important food-borne intoxications. Here, we describe the complete genome sequence of bacteriophage Deep-Blue, which is able to infect emetic strains of B. cereus. Deep-Blue is a 159-kb myophage of the Bastille-like group within the Spounavirinae.
Highlights
The Bacillus cereus emetic pathotype is responsible for important food-borne intoxications
We report the complete genome of bacteriophage Deep-Blue, a newly isolated myovirus infecting emetic strains of B. cereus
Myovirus Deep-Blue was isolated from an agricultural soil collected in Gembloux (Belgium) through sample enrichment using a mixture of emetic B. cereus strains, followed by three singleplaque purification steps using the emetic Bacillus weihenstephanensis strain BtB2-4 [4] as host
Summary
The Bacillus cereus emetic pathotype is responsible for important food-borne intoxications. We report the complete genome of bacteriophage Deep-Blue, a newly isolated myovirus infecting emetic strains of B. cereus. Myovirus Deep-Blue was isolated from an agricultural soil collected in Gembloux (Belgium) through sample enrichment using a mixture of emetic B. cereus strains, followed by three singleplaque purification steps using the emetic Bacillus weihenstephanensis strain BtB2-4 [4] as host. Morphology was determined using transmission electron microscopy (Mica Technology Platform, UCL).
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