Abstract
ABSTRACTBacillus subtilis J-5 was isolated from tomato rhizosphere soil and exhibited strong inhibitory activity against Botrytis cinerea. To shed light on the molecular mechanism underlying the biological control on phytopathogens, the whole genome of this strain was sequenced. Genes encoding antimicrobial compounds and the regulatory systems were identified in the genome.
Highlights
Bacillus subtilis J-5 was isolated from tomato rhizosphere soil and exhibited strong inhibitory activity against Botrytis cinerea
Whole-genome sequencing of strain J-5 was performed on the PacBio RSII sequencing platform at Novogene (Beijing, China)
The complete genome sequence of B. subtilis J-5 is characterized by a circular chromosome of 4,117,900 bp, with a mean GϩC content of 46.11%
Summary
Bacillus subtilis J-5 was isolated from tomato rhizosphere soil and exhibited strong inhibitory activity against Botrytis cinerea. The volatile substances of B. subtilis J-5 were demonstrated to strongly inhibit the growth of B. cinerea in vitro. To gain more knowledge on the genetic equipment of this bacterium and provide more insight into the mechanism by which this bacterium plays its biocontrol roles, we sequenced and annotated the complete genome sequence of this strain. Whole-genome sequencing of strain J-5 was performed on the PacBio RSII sequencing platform at Novogene (Beijing, China).
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